Anti-Homer1 (L113/130) Recombinant Chicken Chimeric mAb

Our chicken Anti-Homer1 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L113/130. It detects human, mouse, and rat Homer1, and is purified by affinity chromatography. It is great for use in AT, IHC, ICC, WB.

Product Specifications

Background

Homer Scaffold Protein 1 is encoded by the gene HOMER1. Homer1 is a member of the Homer family of dendritic proteins. Homer1 is a postsynaptic density scaffolding protein which binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1, and SHANK3. HOMER1 is expressed in the skeletal muscle, heart, and brain. Diseases associated with HOMER1 include Phelan-Mcdermid Syndrome, bipolar disorder, autism, fragile x syndrome, and pervasive developmental disorder.

Synonyms

Homer protein homolog 1 (Homer-1) (VASP/Ena-related gene up-regulated during seizure and LTP 1) (Vesl-1)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Homer1 Vesl1

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 121-363 (C-terminus) of mouse Homer1 (accession number Q9Z2Y3) produced recombinantly in E. Coli

Immunogen Type

Mouse

Target

Homer1

Clonality

Recombinant

Clone

L113/130

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA, AT

Field of Research

Cell Markers Neuronal Markers Synapse Markers Post-synaptic Markers Scaffold Proteins

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L113/130 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L113/130 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

50 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:100

WB Dilution

1:500-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:100

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.