Anti-CALB1/calbindin (L109/57) Recombinant Chicken Chimeric mAb

Our chicken Anti-CALB1/calbindin recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L109/57. It detects human, mouse, and rat CALB1/calbindin, and is purified by affinity chromatography. It is great for use in AT, ICC, IHC, WB.

Product Specifications

Background

Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993) . Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014) .

Short Description

Synonyms

Calbindin (Calbindin D28) (D-28K) (Vitamin D-dependent calcium-binding protein, avian-type)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

CALB1 CAB27

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1-261 (full-length) of human CALB1 (accession number P05937) produced recombinantly in E. Coli

Immunogen Type

Human

Target

CALB1/calbindin

Clonality

Recombinant

Clone

L109/57

Conjugation

Unconjugated

Applications

AT, ELISA, ICC, IHC, WB

Field of Research

Cell Markers Neuronal Markers

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of our NeuroMab clone L109/57 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L109/57 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

30 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:250

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.