Anti-VAChT (N425/45) Recombinant Chicken Chimeric mAb

Our Anti-VAChT chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N425/45. It detects mouse and rat VAChT, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Product Specifications

Background

Vesicular acetylcholine transporter (VaCht), or Solute Carrier Family 18 Member A3 is encoded by the gene Slc18a3 and is a member of the vesicular amine transporter family. VaCht transports acetylcholine into secretory vesicles for release into the extracellular space. VaCht is expressed in major cholinergic cell groups in the peripheral and central cholinergic nervous systems. Diseases associated with SLC18A3 include Myasthenic Syndrome and Presynaptic and Presynaptic Congenital Myasthenic Syndromes.

Short Description

Synonyms

Vesicular acetylcholine transporter (VAChT) (rVAT) (Solute carrier family 18 member 3)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Slc18a3 Vacht Vat

Host

Chicken

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT (accession number Q62666) produced recombinantly in E. Coli

Immunogen Type

Rat

Target

VAChT

Clonality

Recombinant

Clone

N425/45

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Transporter Proteins

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N425/45 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N425/45 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

60 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.