Anti-Beta4-Spectrin (N393/76) Recombinant Chicken Chimeric mAb

Our chicken Anti-Beta4-spectrin recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N393/76. It detects human, mouse, and rat Beta4-spectrin and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Product Specifications

Background

Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND) .

Short Description

Synonyms

Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

SPTBN4 KIAA1642 SPTBN3

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (accession number Q9H254) produced recombinantly in E. Coli

Immunogen Type

Human

Target

Beta4-spectrin

Clonality

Recombinant

Clone

N393/76

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Markers Neuronal Markers Axonal Markers Scaffold Proteins

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N393/76 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N393/76 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

>200 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:250

WB Dilution

1:1000

Specificity

Does not cross-react with other Beta-spectrins

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Uniprot Accession Number

Q9H254

Physical Properties

Liquid

Isotype

IgY

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