Anti-Arl13b (N295B/66) Recombinant Chicken Chimeric mAb

Our chicken Anti-Arl13b recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N295B/66. It detects human, mouse, non-human primate, rat, and zebrafish Arl13b and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

Product Specifications

Background

ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1. Arl13B is a small GTPase with both N-terminal and C-terminal guanine nucleotide-binding motifs. Arl13B is localized to the membrane of cilia and expressed in Neuroepithelial cells and developing radial glia of the developing cerebral cortex. Mutations in this gene are the cause of Joubert syndrome 8.

Short Description

Synonyms

ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) (Protein hennin)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Arl13b Arl2l1

Host

Chicken

Reactivity

Human, Mouse, Non-Human Primate, Rat, and Zebrafish

Immunogen

Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (accession number Q640N2) produced recombinantly in E. Coli

Immunogen Type

Mouse

Target

Arl13b

Clonality

Recombinant

Clone

N295B/66

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Rare Diseases

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N295B/66 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N295B/66 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

50 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:500

WB Dilution

1:1000

Specificity

Does not cross-react with Arl13a (based on KO validation results)

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.