Anti-GFAP (N206A/8) Recombinant Chicken Chimeric mAb

Our chicken Anti-GFAP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N206A/8. It is KO validated, detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP, and is purified by affinity chromatography. It is great for use in ICC, IHC, WB.

Product Specifications

Background

Glial fibrillary acidic protein (GFAP) is an intermediate filament with many different isoforms, and acts as a structural protein in the cytoskeleton. It is expressed in astrocytes in brain and is often used as an astrocyte marker. GFAP is believed to be involved in many CNS processes including cell communication and mitosis. GFAP has been found to be involved in Alexander disease and Wernicke's encephalopathy.

Short Description

Synonyms

Glial fibrillary acidic protein (GFAP)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

GFAP

Host

Chicken

Reactivity

Drosophila, Guinea Pig, Human, Mouse, Polar Bear, and Rat

Immunogen

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (accession number P14136)

Immunogen Type

Human

Target

GFAP

Clonality

Recombinant

Clone

N206A/8

Conjugation

Unconjugated

Applications

ELISA, ICC, IHC, WB

Field of Research

Cell Markers Glial and Schwann Cell Markers Astrocyte Markers

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of our NeuroMab clone N206A/8 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N206A/8 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

50 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:500

WB Dilution

1:1000

Specificity

Cross-reacts with GFAP-R416W and other GFAP mutant proteins. Does not cross-react with other proteins (based on KO validation results) .

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.