Anti-Olig1 (N149/25) Recombinant Chicken Chimeric mAb

Our Anti-Olig1 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N149/25. It detects mouse Olig1, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Product Specifications

Background

Oligodendrocyte transcription factor 1, Basic Domain, Helix-Loop-Helix Protein, Class B, 6 or Class E Basic Helix-Loop-Helix Protein 21 is encoded by the gene Olig1. Olig1 is a transcription factor and a member of the basic helix-loop-helix (bHLH) factor family. Olig1 promotes formation and maturation of oligodendrocytes (oligodendrogensis) . Olig1 is expressed in spinal cord and brain during development, and by oligodendrocytes after late embryonic development. Olig1 is a useful marker for Oligodendrocyte staining. Diseases associated with this gene include Oligodendroglioma and Grade Iii Astrocytoma.

Short Description

Synonyms

Oligodendrocyte transcription factor 1 (Oligo1) (Oligodendrocyte-specific bHLH transcription factor 1)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Olig1

Host

Chicken

Reactivity

Mouse

Immunogen

Fusion protein amino acids 1-67 (N-terminus) of mouse Olig1 (accession number Q9JKN5) produced recombinantly in E. Coli

Immunogen Type

Mouse

Target

Olig1

Clonality

Recombinant

Clone

N149/25

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Markers Glial and Schwann Cell MarkersOligodendrocyte MarkersNeurogenesis and Development Transcription Regulation

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N149/25 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N149/25 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

30 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.