Anti-RGS14 (N133/21) Recombinant Chicken Chimeric mAb

Our Anti-RGS14 chicken monoclonal primary antibody from Aves Labs is produced in-house from hybridoma clone N133/21. It is KO validated, detects mouse and rat RGS14, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Product Specifications

Background

RGS14 is a member of the RGS family (regulator of G-protein signaling) and is highly expressed in the prefrontal cortex. Hallmark domains of RGS14 include a RGS domain, two Raf-like Ras-binding domains and a GoLoco motif. RGS14 inactivates G protein α subunits. Alterations in this protein have been implicated in Schizophrenia and Alzheimer’s disease.

Short Description

Our Anti-RGS14 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N133/21. It is KO validated, detects mouse and rat RGS14, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Regulator of G-protein signaling 14 (RGS14)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Rgs14

Host

Chicken

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-544 (full-length) of rat RGS14 (accession number O08773) produced recombinantly in E. Coli

Immunogen Type

Rat

Target

RGS14

Clonality

Recombinant

Clone

N133/21

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Cell Signaling Scaffold Proteins

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N133/21 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N133/21 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

60 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:500

WB Dilution

1:1000

Specificity

Does not react with RGS12

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.