Anti-Ankyrin-G (N106/36) (Staining) Recombinant Chicken Chimeric mAb

Our chicken Anti-Ankyrin-G (Staining) recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N106/36. It detects feline, hamster, human, mouse, non-human primate, and rat Ankyrin-G (Staining) and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Product Specifications

Background

Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS) . Variants in Ankyrin G have been linked to bipolar disorder.

Short Description

Synonyms

Ankyrin-3 (ANK-3) (Ankyrin-G)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

ANK3

Host

Chicken

Reactivity

Feline, Hamster, Human, Mouse, Non-Human Primate, and Rat

Immunogen

Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli

Immunogen Type

Human

Target

Ankyrin-G (Staining)

Clonality

Recombinant

Clone

N106/36

Conjugation

Unconjugated

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Cell Markers Neuronal Markers Axonal Markers Scaffold Proteins

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N106/36 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N106/36 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

>200 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.