Anti-GluN2B/NR2B Glutamate Receptor (N59/20) Recombinant Chicken Chimeric mAb

Our chicken Anti-GluN2B/NR2B glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from clone N59/20. It detects human, mouse, and rat GluN2B/NR2B glutamate receptor and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Product Specifications

Background

GluN2B/NR2b glutamate receptor, also known as Glutamate/N-methyl D-aspartate/NMDA receptor subtype 2B, is a member of the NMDA receptor family. NMDA receptors are expressed throughout the CNS in postsynaptic cell membranes. GluN2B is a major NMDAR subtype found in cortical and hippocampal regions of the adult brain. NMDA receptors are involved with memory and learning. Mutations in the gene encoding GluN2B are associated with autism, LennoxGastaut and West Syndromes.

Short Description

Synonyms

Glutamate receptor ionotropic, NMDA 2B (GluN2B) (Glutamate [NMDA] receptor subunit epsilon-2) (N-methyl D-aspartate receptor subtype 2B) (NMDAR2B) (NR2B)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Grin2b

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (accession number Q00960) produced recombinantly in E. Coli

Immunogen Type

Rat

Target

GluN2B/NR2B glutamate receptor

Clonality

Recombinant

Clone

N59/20

Conjugation

Unconjugated

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Neurotransmitter Receptors

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N59/2 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N59/2 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

166 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:500

WB Dilution

1:500

Specificity

Does not cross-react with GluN2A/NR2A

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.