Anti-VGlut1 (N28/9) Recombinant Chicken Chimeric mAb

Our chicken Anti-VGlut1 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N28/9. It detects fish, human, mouse, and rat VGlut1, and is purified by affinity chromatography. It is great for use in Flow, IHC, ICC, WB.

Product Specifications

Background

Vesicular glutamate transporter 1 (Vglut1) is encoded by the gene Slc17a7 and belongs to the vesicular glutamate transporter family. Vglut1 is expressed in glutamatergic neurons in brain regions including the cerebellum, cerebral cortex and hippocampus at synaptic vesicles. Isoform 2 is expressed in retinal. Vglut1 functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut1 has been implicated in several neurological and psychiatric diseases including Alzheimer’s disease, Parkinson’s disease, epilepsy and schizophrenia.

Synonyms

Vesicular glutamate transporter 1 (VGluT1) (Brain-specific Na (+) -dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 7)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

Slc17a7 Bnpi Vglut1

Host

Chicken

Reactivity

Fish, Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli

Immunogen Type

Rat

Target

VGlut1

Clonality

Recombinant

Clone

N28/9

Conjugation

Unconjugated

Applications

WB, IHC, ICC, Flow, ELISA

Field of Research

Cell Markers Neuronal Markers Synapse Markers Pre-synaptic Markers

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N28/9 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N28/9 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

52 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:100-1:200

WB Dilution

1:500-1000

Specificity

Does not cross-react with VGlut2 or VGlut3

ICC Dilution

1:100-1:200

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.