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Anti-Pink1 PTEN Induced Putative Kinase 1 (N4/15) Recombinant Chicken Chimeric mAb

Our Anti-Pink1 PTEN induced putative kinase 1 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N4/15. It detects human, mouse, and rat Pink1 PTEN induced putative kinase 1, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Product Specifications

Background

PTEN Induced Kinase 1 is encoded by the gene PINK1. PINK1 is a member of the protein kinase superfamily, Ser/Thr protein kinase family. PINK1 is a serine/threonine protein kinase that protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. PINK1 is highly expressed in the heart, skeletal muscle, and testis. Diseases associated with PINK1 include Parkinson Disease 6, Autosomal Recessive Early-Onset and Parkinson Disease 2, Autosomal Recessive Juvenile.

Short Description

Our Anti-Pink1 PTEN induced putative kinase 1 chicken recombinant primary antibody from Aves Labs is produced in-house from hybridoma clone N4/15. It detects human, mouse, and rat Pink1 PTEN induced putative kinase 1, and is purified by affinity chromatography. It is great for use in WB, IHC, ICC, ELISA.

Synonyms

Serine/threonine-protein kinase PINK1, mitochondrial (EC 2.7.11.1) (BRPK) (PTEN-induced putative kinase protein 1)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

PINK1

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Fusion protein amino acids 112-496 of human Pink1 (accession number NP_115785) produced recombinantly in E. Coli

Immunogen Type

Human

Target

Pink1 PTEN induced putative kinase 1

Clonality

Recombinant

Clone

N4/15

Conjugation

Unconjugated

Applications

WB, IHC, ICC, ELISA

Field of Research

Mitochondrial Biology Neurodegenerative Disease Parkinson's Disease

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N4/15 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N4/15 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

63 kDa

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Product Datasheet

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/78-060__datasheet.pdf

IHC Dilution

1:200-1:1000

Specificity

No cross-reactivity reported

ICC Dilution

1:200-1:1000

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when tested by Western Blot.

Uniprot Accession Number

Q9BXM7

Physical Properties

Liquid

Isotype

IgY

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