Anti-PSD-95 (K28/43) Recombinant Chicken Chimeric mAb

Our chicken Anti-PSD 95 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone K28/43. It is KO validated, detects chicken, human, mouse, non-human primate, and rat PSD 95, and is purified by affinity chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003) . Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Synonyms

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

DLG4 PSD95

Host

Chicken

Reactivity

Chicken, Human, Mouse, Non-Human Primate, and Rat

Immunogen

Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli

Immunogen Type

Human

Target

PSD 95

Clonality

Recombinant

Clone

K28/43

Conjugation

Unconjugated

Applications

WB, IHC, ICC, IP, ELISA

Field of Research

Cell Markers Neuronal Markers Synapse Markers Post-synaptic Markers Scaffold Proteins

Assay Protocol

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K28/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K28/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Concentration

1 mg/mL

Format

Purified by affinity chromatography

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Molecular Weight

95-110 kDa (varies with cell background due to phosphorylation)

Shipping Conditions

Shipped on Ice Packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

IHC Dilution

1:500

WB Dilution

1:1000

Specificity

No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.