Anti-SQSTM1 Antibody

Our Anti-SQSTM1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat SQSTM1, and is affinity-purified IgY. It is great for use in ICC, IHC, WB.

Product Specifications

Background

Sequestosome 1 (SQSTM1), also known as p62, is a multifunctional adaptor protein involved in autophagy, cell signaling, and protein degradation. It plays a key role in selectively targeting ubiquitinated proteins for degradation via the autophagy-lysosome pathway and is also involved in NF-κB signaling, oxidative stress responses, and bone metabolism. Mutations in SQSTM1 are strongly associated with Paget’s disease of bone and have been implicated in neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and certain cancers. Due to its role in cellular homeostasis, SQSTM1 remains a significant focus in research on autophagy-related diseases and targeted therapies.

Short Description

Our Anti-SQSTM1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat SQSTM1, and is affinity-purified igy. It is great for use in ICC, IHC, WB.

Synonyms

Sequestosome-1, ORCA, OSIL, P62, Autophagy Receptor P62

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

SQSTM1

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Human SQSTM1 full sequence

Immunogen Type

Human

Target

SQSTM1

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, IHC, WB

Field of Research

Cell Markers Lysosome Markers

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Concentration

200 µg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

Calculated - 48 kDa; Observed - 62 kDa

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

IHC Dilution

1:100 - 1:200

WB Dilution

1:5000 - 1:10000

Specificity

No cross-reactivity reported

ICC Dilution

1:100 - 1:200

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe HeLa cell lysate.