Anti-NCAM1 Antibody

Our Anti-NCAM1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat NCAM1, and is affinity-purified IgY. It is great for use in ICC, IHC, WB.

Product Specifications

Background

Neural cell adhesion molecule 1 (NCAM1), also known as CD56, is a glycoprotein first characterized for its role in neural development and cell-cell adhesion. It is widely expressed in neurons, glial cells, skeletal muscle, and immune cells, including natural killer (NK) cells. NCAM1 plays a crucial role in neuron-neuron adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. It interacts with fibroblast growth factor receptors (FGFRs) to influence intracellular signaling pathways. NCAM1 has been implicated in neurological disorders such as schizophrenia and bipolar disorder, as well as in certain immune-related diseases. Due to its role in neural plasticity and immune function, NCAM1 remains a key target in research on brain repair, psychiatric disorders, and immunotherapy.

Short Description

Our Anti-NCAM1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat NCAM1, and is affinity-purified igy. It is great for use in ICC, IHC, WB.

Synonyms

NCAM, CD56, CD56 Antigen

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

NCAM1

Host

Chicken

Reactivity

Human, Mouse, and Rat

Immunogen

Human NCAM1 1-718 (Extracellular region)

Immunogen Type

Human

Target

NCAM1

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, IHC, WB

Field of Research

Cell Adhesion Molecules

Assay Protocol

Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.

Concentration

200 µg/mL

Format

Affinity-Purified IgY

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Molecular Weight

Calculated - 95 kDa; Observed - 120 - 180 kDa

Shipping Conditions

Ice Packs

Storage Conditions

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

IHC Dilution

1:200 - 1:500

WB Dilution

1:2000 - 1:10000

Specificity

No cross-reactivity reported

ICC Dilution

1:200 - 1:500

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.