Vimentin Antibody
Mouse monoclonal antibody to Vimentin
Product Specifications
UniProt
P08670
Reactivity
Gallus, Human, Mouse, Porcine, Rat
Target
Vimentin
Clonality
Monoclonal
Clone
VI-10
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Immunohistochemistry (paraffin sections) : Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.Western blotting: Recommended dilution: 1-2 μg/ml.
Tested Applications
ICC, IHC-P, IP, WB
Preservative
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Isotype
Mouse IgM
Entrez
7431
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