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Gamma-Tubulin Antibody

Mouse Monoclonal to gamma Tubulin.

Product Specifications

Product Name Alternative

TUBG

UniProt

P23258

Reactivity

Bovine, Gallus, Human, Mouse, Plant, Porcine, Protozoa, Rat

Immunogen

C-terminal peptide of gamma-tubulin counjugated to KLH.

Target

Gamma-Tubulin

Clonality

Monoclonal

Clone

TU-30

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunocytochemistry: Recommended dilution: 1-2 μg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.Western blotting: Recommended dilution 1-2 μg/ml, reducing conditions.

Tested Applications

FC, ICC, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Isotype

Mouse IgG1

Entrez

7283

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