CD3 Antibody
Mouse monoclonal antibody to CD3
Product Specifications
Product Name Alternative
CD3E, T3E, TCRE
UniProt
P07766
Reactivity
Human
Immunogen
Human thymocytes and T lymphocytes.
Target
CD3
Clonality
Monoclonal
Clone
MEM-57
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunoprecipitation: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7) . Functional application: The antibody MEM-57 has strong mitogenic effect on peripheral T lymphocytes, it reacts strongly with gamma/delta T lymphocytes. Flow cytometry: Recommended dilution: 1-4 μg/ml; positive control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.
Tested Applications
FA, FC, IP
Preservative
Phosphate buffered saline (PBS), pH 7.4
Isotype
Mouse IgG2a kappa
Entrez
916
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