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CD45 Antibody

Mouse monoclonal antibody to CD45

Product Specifications

Product Name Alternative

LCA, T200, LY5, B220, GP180, TPC

UniProt

P08575

Reactivity

Human

Immunogen

Human thymocytes and T lymphocytes.

Target

CD45

Clonality

Monoclonal

Clone

MEM-28

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunohistochemistry (paraffin sections) : No pre-treatment of tissue sections is needed. Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used. Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa) . Flow cytometry: Recommended dilution: 1 μg/ml.

Tested Applications

FC, ICC, IHC-P, IP, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Isotype

Mouse IgG1

Entrez

5788

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