CD11b Antibody
Mouse monoclonal antibody to CD11b
Product Specifications
Product Name Alternative
Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A
UniProt
P11215
Reactivity
Human
Immunogen
Human granulocytes
Target
CD11b
Clonality
Monoclonal
Clone
MEM-174
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes. Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants. Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns) . The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads) . It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.
Tested Applications
FC, IP
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG2a
Entrez
3684
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