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GAPDHS Antibody

Mouse Monoclonal to Intra-Acrosomal Protein.

Product Specifications

Product Name Alternative

SGAPDH, HSD-35

UniProt

O14556

Reactivity

Human, Porcine

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Target

GAPDHS

Clonality

Monoclonal

Clone

Hs-8

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential. Flow cytometry: Recommended dilution: 3-12 μg/ml. Intraacrosomal staining.

Tested Applications

FC, ICC, WB

Preservative

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Isotype

Mouse IgM

Entrez

26330

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