VCP Antibody
Mouse Monoclonal to Intra-Acrosomal Protein.
Product Specifications
Product Name Alternative
TERA, CDC48
UniProt
P55072
Reactivity
Human, Mouse
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Target
VCP
Clonality
Monoclonal
Clone
Hs-14
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods) .
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunocytochemistry: Recommended dilution: 10 μg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought) . Flow cytometry: Intraacrosomal staining; recommended dilution: 3-12 μg/ml.
Tested Applications
FC, ICC, WB
Preservative
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Isotype
Mouse IgM
Entrez
7415
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