Cytokeratin 10/13 Antibody
Mouse Monoclonal to Cytokeratin 10/13.
Product Specifications
Product Name Alternative
K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13
Reactivity
Feline, Human
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium.
Target
Cytokeratin 10/13
Clonality
Monoclonal
Clone
DE-K13
Conjugation
Unconjugated
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunohistochemistry (paraffin sections) : Standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. Immunohistochemistry (frozen sections) : Use at an assay dependent dilution. Western blotting: recommended dilution: 1-3 μg/ml.
Tested Applications
IHC-Fr, IHC-P, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG2a
Entrez
35
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