Cytokeratin 18 Antibody
Mouse monoclonal antibody to Cytokeratin 18
Product Specifications
Product Name Alternative
K18, CK18, CYK18, KRT18
UniProt
P05783
Reactivity
Human
Immunogen
Human breast carcinoma cell line PMC-42.
Target
Cytokeratin 18
Clonality
Monoclonal
Clone
DA-7
Conjugation
Unconjugated
Field of Research
Epigenetics, Infectious Diseases
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blotting: Recommended dilution: 1-2 μg/ml.Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: Positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line.ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. orb43702) .
Tested Applications
ELISA, ICC, IHC-P, IP, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG1
Entrez
3875
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