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Cyclin D1 Antibody

Mouse Monoclonal to Cyclin D1.

Product Specifications

Product Name Alternative

CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

UniProt

P24385

Reactivity

Human, Rat

Immunogen

Purified cyclin D1 protein

Target

Cyclin D1

Clonality

Monoclonal

Clone

CD1.1

Conjugation

Unconjugated

Field of Research

Cancer, DNA Damage, Epigenetics

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunohistochemistry (frozen sections) : Recommended dilution: 2 μg/ml; positive tissue: colon.Western blotting: Recommended dilution: 1 μg/ml.Immunocytochemistry: Recommended dilution: 1 μg/ml. Immunoprecipitation: Recommended dilution: 1 μg/ml.Immunohistochemistry (paraffin sections) : Heat treatment, sodium citrate buffer pH 6.0.Flow cytometry: Recommended dilution: 4-10 μg/ml. Intracellular staining.

Tested Applications

ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Isotype

Mouse IgG1

Entrez

595

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