Alpha-Fetoprotein Antibody
Mouse monoclonal antibody to Alpha 1 Fetoprotein
Product Specifications
Product Name Alternative
AFP, FETA, HPAFP
UniProt
P02771
Reactivity
Human
Immunogen
Purified alpha-Fetoprotein
Target
Alpha-Fetoprotein
Clonality
Monoclonal
Clone
AFP-01
Conjugation
Unconjugated
Field of Research
Cardiovascular Disease, Epigenetics
Purification
Purified by protein-A affinity chromatography.
Concentration
1 mg/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100) .Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents) . Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
Tested Applications
ELISA, ICC, IP, WB
Preservative
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Isotype
Mouse IgG1
Entrez
174
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