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C-Myc tag Antibody

Mouse Monoclonal to c-Myc.

Product Specifications

Product Name Alternative

BHLH, MRTL, MYCC

UniProt

P01106

Reactivity

All, Human

Immunogen

Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.

Target

C-Myc tag

Clonality

Monoclonal

Clone

9E10

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified by protein-A affinity chromatography.

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunohistochemistry (paraffin sections) : Recommended dilution: 5-10 μg/ml; positive tissue: perfused brain sections, liver, spleen. Immunoprecipitation: Recommended dilution: 1-5 μg/ml; this antibody is not suitable for immunoprecipitation of native c-Myc protein. Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 μg/ml.Western blotting: Recommended dilution: 0,5-2 μg/ml; positive control: c-Myc tagged protein.

Tested Applications

ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB

Preservative

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Isotype

Mouse IgG1

Entrez

4609

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