Human BRCA2 (Breast Cancer Susceptibility Protein 2) ELISA Kit

BRCA2; BRCC2; FACD; FAD; FAD1; FANCD; FANCD1; Breast Cancer 2, Early Onset; Breast Cancer Type 2 Susceptibility Protein; Fanconi anemia group D1 protein

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BRCA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BRCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BRCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BRCA2. You can calculate the concentration of Human BRCA2 in the samples by comparing the OD of the samples to the standard curve.