Human TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A) ELISA Kit

CD266; TNFRSF12-A; FN14; TWEAKR; Tumour Necrosis Factor Related Weak Inducer Of Apoptosis; Fibroblast growth factor-inducible immediate-early response protein 14

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF12A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFRSF12A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFRSF12A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNFRSF12A. You can calculate the concentration of Human TNFRSF12A in the samples by comparing the OD of the samples to the standard curve.