Rabbit NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit NT-ProBNP. During the reaction, Rabbit NT-ProBNP in the sample or standard competes with a fixed amount of Rabbit NT-ProBNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rabbit NT-ProBNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rabbit NT-ProBNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.