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Human Cortisol (Cortisol) ELISA Kit

Product Specifications

Cross Reactivity

The kit detected Human Cortisol in the samples and no significant cross-species reactions were found

Target

Cortisol

Type

Colormetric

Field of Research

Signal transduction, Metabolism

Detection Method

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. During the reaction, Human Cortisol in the sample or standard competes with a fixed amount of Human Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Type

Competitive

Assay Performance Time

1.5h

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

12.35-1000ng/mL

Precision

CV<10%

Recovery

80%-120%

Sensitivity

5.15ng/mL

Storage Conditions

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

Species

Human

Curated Selection

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