GMP Recombinant Human Wnt-3a (hFc Tag)
Product Specifications
Background
Wnt surrogate is a Wnt pathway agonists and related compositions, which may be used in any of a variety of therapeutic methods for the treatment of diseases.
Product Name Alternative
MGC119418; MGC119419; MGC119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a
Accession Number
NP_149122
Host
Mammalian cell
Target
Wnt-3a (hFc Tag)
Tag
HFc Tag
Endotoxin
≤10 EU/mg
Purity
Greater than 95% as determined by reducing SDS-PAGE
Activity
Measured by its ability to induce Topflash reporterBioactivity in CHO-K1 cells. The ED50 for this effect is <30ng/ml.
Form
Lyophilized
Buffer
PBS, pH7.4
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
58.2 kDa
Shipping Conditions
The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.
Construct
Recombinant Human Wnt-3a (hFc Tag) is produced by our Mammalian cell expression system and the target gene encoding Ser19-Lys352 with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330) is expressed.
Species
Human
Curated Selection
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