GMP Recombinant Human TGF-β3

Product Specifications

Background

Transforming growth factor beta 3 (TGFB3) is a member of a TGF -β superfamily which is defined by theirstructural and functional similarities. TGFB3 is secreted as a complex with LAP. This latent form of TGFB3becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin -1, and a subset ofintegrins. It binds with high affinity to TGF- β RII, a type II serine/threonine kinase receptor. TGFB3 is involved incell differentiation, embryogenesis and development.It is believed to regulate molecules involved in cellularadhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-β3, mammals develop a deformity known as a cleft palate.

Product Name Alternative

Transforming growth factor beta-3; TGFB3; TGF-beta-3; Latency-associated peptide; LAP

Accession Number

P10600

Host

Mammalian cell

Target

TGF-β3

Tag

Tag free

Endotoxin

≤10 EU/mg

Purity

Greater than 95% as determined by reducing SDS-PAGE

Activity

Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2ng/mL.

Form

Lyophilized

Buffer

100 mM Glycine, 150 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 4.0

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

13 kDa

Shipping Conditions

The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.