GMP Recombinant Human PDGF-BB
Product Specifications
Background
Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
Product Name Alternative
PDGFBB; PDGF-BB
Accession Number
P01127
Host
Yeast
Target
PDGF-BB
Tag
Tag free
Endotoxin
≤10 EU/mg
Purity
Greater than 95% as determined by reducing SDS-PAGE
Activity
Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤5 ng/mL.
Form
Lyophilized
Buffer
100mM Citrate sodium citrate, 5% Mannitol 0.1% Trehalose pH 6.5
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
12.2 kDa
Shipping Conditions
The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.
Construct
Recombinant Human PDGF-BB is produced by our Yeast expression system and the target gene encoding Ser82-Thr190 is expressed.
Species
Human
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