GMP Recombinant Human IL-23

Product Specifications

Background

Interleukin-23 (IL-23) is a heterodimeric cytokine that belongs to the IL-12 cytokine family. It is composed of two subunits, p19 and p40, with the p40 subunit being shared with IL-12. IL-23 is primarily secreted by activated macrophages and dendritic cells located in peripheral tissues such as skin, intestinal mucosa, and lung. This cytokine plays a crucial role in the development and maintenance of autoimmune inflammation, particularly through its ability to induce IL-17 production, which is a unique role compared to IL-12.

Product Name Alternative

SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2

Accession Number

P29460 (P40), AAG37232 (P19)

Host

Mammalian cell

Target

IL-23

Tag

Tag free

Endotoxin

≤10 EU/mg

Purity

Greater than 95% as determined by reducing SDS-PAGE

Activity

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 μg/mL.

Form

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

55.0 kDa (IL-23singlechain) ;36.5 kDa (p40) &18.5 kDa (p19)

Shipping Conditions

The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.