GMP Recombinant Human IL-13

Product Specifications

Background

Interleukin-13 is also known as IL-13. It is a protein that in humans is encoded by the IL13 gene. Interleukin-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells.It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils.

Product Name Alternative

Interleukin-13; IL-13

Accession Number

P35225

Host

Mammalian cell

Target

IL-13

Tag

Tag free

Endotoxin

≤10 EU/mg

Purity

Greater than 95% as determined by reducing SDS-PAGE

Activity

Measured in a cell proliferation assay using TF-1 cells. The ED50 ≤1.0ng/ml, corresponding to a specific Bioactivity of ≥1x106 U/mg.

Form

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

12.3 kDa

Shipping Conditions

The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.