GMP Recombinant Human IL-7
Product Specifications
Background
Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT, which regulate lymphocyte survival, glucose uptake, proliferation, and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.
Product Name Alternative
Interleukin-7; IL-7; IL7
Accession Number
P13232
Host
Mammalian cell
Target
IL-7
Tag
Tag free
Endotoxin
≤10 EU/mg
Purity
Greater than 95% as determined by reducing SDS-PAGE
Activity
Measured in a cell proliferation assay using NALM-6 human pre-B cells. The ED50 for this effect is ≤30ng/ml.
Form
Lyophilized
Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
17.3 kDa
Shipping Conditions
The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.
Product Datasheet
https://assets.enkilife.com/files/GMP/PCH90031_EnkiLife_EN.pdf
Construct
Recombinant Human IL-7 is produced by our Mammalian cell expression system and the target gene encoding Asp26-His177 is expressed.
Species
Human
Curated Selection
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