GMP Recombinant Human FGF-7/KGF

Product Specifications

Background

Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.

Product Name Alternative

Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF

Accession Number

P21781

Host

Mammalian cell

Target

FGF-7/KGF

Tag

Tag free

Endotoxin

≤10 EU/mg

Purity

Greater than 95% as determined by reducing SDS-PAGE

Activity

Measured by its ability to induce lL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is ≤1 μg/mL.

Form

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

18.8 kDa

Shipping Conditions

The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.