GMP Recombinant Human EGF
Product Specifications
Background
Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.
Product Name Alternative
Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Accession Number
P01133
Host
Mammalian cell
Target
EGF
Tag
Tag free
Endotoxin
≤10 EU/mg
Purity
Greater than 95% as determined by reducing SDS-PAGE
Activity
Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL.
Form
Lyophilized
Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
6.2 kDa
Shipping Conditions
The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.
Construct
Recombinant Human EGF is produced by our Mammalian cell expression system and the target gene encoding Asn971-Arg1023 is expressed.
Species
Human
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