GMP Recombinant Human bFGF/FGF-2
Product Specifications
Background
Fibroblast growth factor 2 (FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial, tumor and other cell types. It involved in developmental processes and regulates differentiation, proliferation, and migration, FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.
Product Name Alternative
Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB
Accession Number
P09038
Host
E coli
Target
BFGF/FGF-2
Tag
Tag free
Endotoxin
≤10 EU/mg
Purity
Greater than 95% as determined by reducing SDS-PAGE
Activity
Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤2.0 ng/mL.
Form
Lyophilized
Buffer
10mM PB,250mM NaCl,5% mannitol and 0.01% Tween 80, pH 7.4
Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
17 kDa
Shipping Conditions
The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.
Construct
Recombinant Human bFGF/FGF-2 is produced by our E coli expression system and the target gene encoding Pro 143-Ser 288 is expressed.
Species
Human
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