GMP Recombinant Human bFGF/FGF-2

Product Specifications

Background

Fibroblast growth factor 2 (FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial, tumor and other cell types. It involved in developmental processes and regulates differentiation, proliferation, and migration, FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase.

Product Name Alternative

Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

Accession Number

P09038

Host

E coli

Target

BFGF/FGF-2

Tag

Tag free

Endotoxin

≤10 EU/mg

Purity

Greater than 95% as determined by reducing SDS-PAGE

Activity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤2.0 ng/mL.

Form

Lyophilized

Buffer

10mM PB,250mM NaCl,5% mannitol and 0.01% Tween 80, pH 7.4

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Molecular Weight

17 kDa

Shipping Conditions

The product is shipped with ice bag. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

36 months at -20°C to -80°C in lyophilized state.6 months at -20°C to -80°C under sterile conditions after reconstitution.7-10 days at 2°C to 8°C under sterile conditions after reconstitution.Please minimize freeze-thaw cycles.