Luciferase (6B8) Mouse Monoclonal Antibody
Product Specifications
Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.bioluminescence, oxidation reduction
Swiss Prot
P08659
Host
Mouse
Reactivity
Firefly
Clonality
Monoclonal
Conjugation
Unconjugated
Applications
WB, ICC/IF
Field of Research
Tags & Cell Markers; Fusion / Marker Proteins; Luciferase; Signal Transduction; Antibodies; firefly luciferase
Purification
Affinity purification
Dilution
WB 1:1000-1:2000, ICC/IF 1:50-1:200
Form
Liquid
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Modification
Unmodified
Molecular Weight
60kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
Curated Selection
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