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Luciferase (6B8) Mouse Monoclonal Antibody

Product Specifications

Background

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.bioluminescence, oxidation reduction

Swiss Prot

P08659

Host

Mouse

Reactivity

Firefly

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

WB, ICC/IF

Field of Research

Tags & Cell Markers; Fusion / Marker Proteins; Luciferase; Signal Transduction; Antibodies; firefly luciferase

Purification

Affinity purification

Dilution

WB 1:1000-1:2000, ICC/IF 1:50-1:200

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Modification

Unmodified

Molecular Weight

60kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Isotype

IgG

Available Sizes

Curated Selection

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