Topoisomerase I Rabbit Polyclonal Antibody
Product Specifications
Background
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 5'-OH DNA strand.
Product Name Alternative
TOP1; DNA topoisomerase 1; DNA topoisomerase I
Gene Name
TOP1
Gene ID
7150
Swiss Prot
P11387
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB, IHC, ICC/IF, FC
Field of Research
Epigenetics and Nuclear Signaling
Purification
Affinity Chromatography
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, ICC/IF 1:50-1:200, FC 1:50-1:100
Form
Liquid
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Unmodified
Molecular Weight
Calculated MW: 91 kDa; Observed MW: 91 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.
Isotype
IgG
Available Sizes
Curated Selection
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