P53 Rabbit Polyclonal Antibody

Product Specifications

Background

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Product Name Alternative

TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Gene Name

TP53

Gene ID

7157

Swiss Prot

P04637

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB, IHC, ICC/IF, ELISA

Field of Research

Cell Biology

Purification

Affinity Purification

Dilution

WB 1:500-1:1000, IHC 1:50-1:100, ICC/IF 1:50-1:200, ELISA 1:5000-1:20000

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Modification

Unmodified

Molecular Weight

Calculated MW: 44 kDa; Observed MW: 53 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Isotype

IgG

Available Sizes

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