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Phospho-Smad3 (Ser425) Rabbit Polyclonal Antibody

Product Specifications

Background

Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad) . Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Product Name Alternative

SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Gene Name

SMAD3

Gene ID

4088

Swiss Prot

P84022

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB, IHC, ICC/IF, ELISA

Field of Research

Signal Transduction

Purification

Affinity Purification

Dilution

WB 1:500-1:1000, IHC 1:50-1:100, ICC/IF 1:50-1:200, ELISA 1:5000-1:20000

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Modification

Phosphorylated

Molecular Weight

Calculated MW: 48 kDa; Observed MW: 58 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Isotype

IgG

Available Sizes

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