CAD (phospho Thr456) Rabbit Polyclonal Antibody

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015], catalytic activity: (S) -dihydroorotate + H (2) O = N-carbamoyl-L-aspartate., catalytic activity:2 ATP + L-glutamine + HCO (3) (-) + H (2) O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate., catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate., cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) ., enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction., function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase) ., miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5) ., online information:Aspartate carbamoyltransferase entry, pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 1/6., pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 2/6., pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 3/6., similarity:Belongs to the ATCase/OTCase family., similarity:Contains 1 glutamine amidotransferase type-1 domain., similarity:Contains 2 ATP-grasp domains., similarity:In the central section; belongs to the DHOase family., subunit:Homohexamer.