Nanog P8 Rabbit Polyclonal Antibody

This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. [provided by RefSeq, Sep 2009], developmental stage:Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level) . Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level) . Expressed in embryonic stem cells (ES) . Expression decreases with ES differentiation., function:May act as a transcription regulator (By similarity) . When overexpressed, promotes cells to enter into S phase and proliferation., function:Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity) . Acts as a transcriptional activator or repressor (By similarity) . Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity) . When overexpressed, promotes cells to enter into S phase and proliferation., miscellaneous:Almost identical to NANOG. There are only one change in the inferred amino acid sequence from 'Gln-253' in NANOG to His-253 in NANOGP8., miscellaneous:Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11) ; except NANOGP8 which is a retrogene., online information:Nanog entry, similarity:Belongs to the Nanog homeobox family., similarity:Contains 1 homeobox DNA-binding domain., subunit:Interacts with SMAD1 and SALL4., tissue specificity:Expressed in osteosarcoma cancer cell line (at protein level) (Probable) . Expressed in tumor uterine cervix, breast and urinary bladder tissues, and also osteosarcoma, hepatoma, and breast adenocarcinoma cancer cell lines., tissue specificity:Expressed in testicular carcinoma and derived germ cell tumors (at protein level) . Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.