DNA pol ι Rabbit Polyclonal Antibody

Catalytic activity:Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1) ., cofactor:Magnesium., domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity., function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity., similarity:Belongs to the DNA polymerase type-Y family., similarity:Contains 1 umuC domain., subcellular location:Accumulates at replication forks after DNA damage., subunit:Binds REV1L (By similarity) . Binds POLH., tissue specificity:Ubiquitous. Highly expressed in testis., catalytic activity:Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1) ., cofactor:Magnesium., domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity., function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity., similarity:Belongs to the DNA polymerase type-Y family., similarity:Contains 1 umuC domain., subcellular location:Accumulates at replication forks after DNA damage., subunit:Binds REV1L (By similarity) . Binds POLH., tissue specificity:Ubiquitous. Highly expressed in testis.