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GAPDH Rabbit Monoclonal Antibody

Product Specifications

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome. [provided by RefSeq, Jan 2014]

Product Name Alternative

G3PD; GAPD; HEL-S-162eP

Gene Name

GAPDH

Gene ID

14433

Swiss Prot

P16858

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey, Rabbit, Chicken, Zebrafish, Rabbit, Xenopus tropicalis, Chinese hamster, E. Escherichia coli

Clonality

Monoclonal

Conjugation

Unconjugated

Applications

WB, IHC, ICC/IF, FC, IP

Purification

Affinity Purification

Dilution

WB 1:1000-1:5000, IHC 1:200-1:2000, ICC/IF 1:200-1:500, FC 1:100-1:200, IP 1:50-1:100

Form

Liquid

Buffer

Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Modification

Unmodified

Molecular Weight

Calculated MW:36 kDa; Observed MW:36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Isotype

IgG

Available Sizes

Curated Selection

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